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Greater than ninety percent of the cells excluded dye in all cases. Similarly, lactate dehydrogenase release was not altered between control and drug treated macrophages. The amount of lactate dehydrogenase released by untreated and drug treated histone deacetylase inhibitor macrophages was lower than 10% of that observed by lysis of manage macrophages. Release of lysozyme, a constitutive solution of macrophages, was not markedly altered by drug remedy. Common protein synthesis by macrophages, as measured by uptake of leucine is shown in fig. 3. Protein synthesis was not appreciably altered by remedy with 2 Lg/nil GST or 0. 1 /xg/ml auranofin. GST reduced leucine incorporation, by lower than 25%, as did thiomalic acid.

The average absolute levels of baseline output of 5 HT in the ventra hippocampus ranged from 54. 6 to 76. 6 finol/20 ju, perfusate. The baseline 5 HT values tended to be slightly elevated within the rats that had received bnUis 8 OH DPAT the day just before the microdialysis experiment. Nonetheless, there were no significant variations in between contro and corresponding 8 OH DPAT pretreated groups. As in untreated IEM 1754 animals, 8OH DPAT challenge caused a BMY 7378 to decrease the ventra hippocampa release of 5 HT. As is evident from the data presented in fig. 3 and table 2, ipsapirone administration resulted in a maximum 70 75% reduction in ventra hippocampa 5 HT output. The overal 5 HT release during the 2 h after injection was suppressed by about 65% by this dose of ipsapirone.

After reflection of the scalp, the skull overlying both substantia nigra and the ventral tegmental area was removed. Extracellular recordings PARP were performed using single barrel micropipettes DA neurons were identified by their location, waveform. firing rate and pattern Electrical signals of spike activity were pa. ssed through a high input impedance amplifier whose output was led into an analog oscilloscope, audio monitor and window discriminator. Unit activity was then converted to an integrated histogram by a rate averaging computer and displayed as spikes per 10 s intervals on a chart recorder. At the end of the chronic studies spontaneously firing DA cells within both SNc and VTA regions were counted by lowering the electrode through a block of tissue which could be reproducibly located from animal to animal Twelve clectrode tracks, in a sequence kept constant from animal to animal, were made in each region.